Strain Passport - Bacillus megaterium

1. Taxonomy

Taxonomy
Domain Bacteria
Phylum Firmicutes
Class Bacilli
Order Bacillales
Family Bacillaceae_H
Genus Bacillus_C
Species Bacillus_C megaterium

2. Assembly Statistics

Assembly Statistics
MAGID MAG25
Size (bp) 4561334
N.contigs 49
GC (%) 38.3
N50 (bp) 158869
Coding Density 85.3
N.CDSs 4633
Genome Completeness
Completeness 99.43
Contamination 0.03
Strain heterogeneity 0.00

3. Metabolism and Function (KEGG)

KEGG Function Annotation

Bacillus megaterium KEGG Functional Classification

KEGG Resistome

name Type Level1 Level2 Description KO
ko01504 Gene variants Aminoglycoside resistance genes N-Acetyltransferases K00662 aacC; aminoglycoside 3-N-acetyltransferase [EC:2.3.1.81] K00662
ko01504 Gene variants Aminoglycoside resistance genes N-Acetyltransferases K18815 aac6-I; aminoglycoside 6’-N-acetyltransferase I [EC:2.3.1.82] K18815
ko01504 Gene variants Aminoglycoside resistance genes Other acetyltransferases K19273 sat4; streptothricin acetyltransferase [EC:2.3.-.-] K19273
ko01504 Gene variants Aminoglycoside resistance genes O-Phosphotransferases K04343 strB; streptomycin 6-kinase [EC:2.7.1.72] K04343
ko01504 Gene variants Tetracycline resistance genes Transporters K18218 tet35; tetracycline resistance efflux pump K18218
ko01504 Gene variants Tetracycline resistance genes Others K18220 tetM, tetO; ribosomal protection tetracycline resistance protein K18220
ko01504 Gene variants Macrolide resistance genes Transporters K18231 msr, vmlR; macrolide transport system ATP-binding/permease protein K18231
ko01504 Gene variants Macrolide resistance genes Others K06880 ereA_B; erythromycin esterase [EC:3.1.1.-] K06880
ko01504 Gene variants Macrolide resistance genes Others K18235 vgb; virginiamycin B lyase [EC:4.2.99.-] K18235
ko01504 Gene variants Macrolide resistance genes Others K18234 vat; virginiamycin A acetyltransferase [EC:2.3.1.-] K18234
ko01504 Gene sets beta-Lactam resistance modules beta-Lactam resistance, Bla system [MD:M00627] K02171 blaI; BlaI family transcriptional regulator, penicillinase repressor K02171
ko01504 Gene sets beta-Lactam resistance modules beta-Lactam resistance, Bla system [MD:M00627] K17836 penP; beta-lactamase class A [EC:3.5.2.6] K17836
ko01504 Gene sets Vancomycin resistance modules Vancomycin resistance, D-Ala-D-Lac type [MD:M00651] K07260 vanY; zinc D-Ala-D-Ala carboxypeptidase [EC:3.4.17.14] K07260
ko01504 Gene sets Vancomycin resistance modules Vancomycin resistance, D-Ala-D-Lac type [MD:M00651] K18346 vanW; vancomycin resistance protein VanW K18346
ko01504 Gene sets Vancomycin resistance modules Vancomycin resistance, D-Ala-D-Ser type [MD:M00652] K18350 vanSC, vanSE, vanSG; two-component system, OmpR family, sensor histidine kinase VanS [EC:2.7.13.3] K18350
ko01504 Gene sets Vancomycin resistance modules Vancomycin resistance, D-Ala-D-Ser type [MD:M00652] K18349 vanRC, vanRE, vanRG; two-component system, OmpR family, response regulator VanR K18349
ko01504 Gene sets Multipdrug resistance modules Multidrug resistance, efflux pump AbcA [MD:M00700] K18907 norG; GntR family transcriptional regulator, regulator for abcA and norABC K18907
ko01504 Gene sets Multipdrug resistance modules Multidrug resistance, efflux pump AbcA [MD:M00700] K18104 abcA, bmrA; ATP-binding cassette, subfamily B, bacterial AbcA/BmrA [EC:7.6.2.2] K18104
ko01504 Gene sets Multipdrug resistance modules Multidrug resistance, efflux pump NorB [MD:M00702] K18907 norG; GntR family transcriptional regulator, regulator for abcA and norABC K18907

KEGG Toxins

name Type Level1 Level2 Description KO
ko02042 Type II toxins: Membrane damaging toxins Pore-forming toxins, Other K03699 tlyC; magnesium and cobalt exporter, CNNM family tlyC; magnesium and cobalt exporter, CNNM family K03699
ko02042 Type II toxins: Membrane damaging toxins Pore-forming toxins, Other K11068 hlyIII; hemolysin III hlyIII; hemolysin III K11068

KEGG Bacterial Virulence

GeneID KO Description PFAM
MAG25_CFLALH_00700 K08303 U32 family peptidase [EC:3.4.-.-] Peptidase family U32 [PF01136.22]…
MAG25_CFLALH_00875 K03596 GTP-binding protein LepA GTP-binding protein LepA C-terminus [PF06421.15]…
MAG25_CFLALH_13625 K14956 6 kDa early secretory antigenic target Proteins of 100 residues with WXG [PF06013.15]…

Carbon Metabolism

Nitrogen Metabolism

4. Expert systems

AMRFinderPlus Antimicrobial Resistance

AMRFinderPlus
locus pscc.product gene_family class subclass
CFLALH_08180 Beta-lactamase bla BETA-LACTAM BETA-LACTAM

Virulence factors (VFDB)

## No VFDB data

5. BacDive

BacDive
Species Bacillus megaterium
ncbi_taxid NA
taxid_matching_level NA
type_strain no
medium_name MEDIUM 29- Brain heart agar
medium_link NA
strain_history NA
gram NA
length NA
width NA
motility NA
oxygen_tolerance NA
spore NA
aggregation_score NA
GC_content NA
antibiotic_resistance_chebi NA
antibiotic_resistance_metabolite NA

6. Publications

7. U.S. Patents

First 10 patents out of 42

patent_number patent_title
10154670 Compositions comprising Bacillus strains and methods of use to suppress the activities and growth of fungal plant pathogens
10602743 Method of inducing drought/salt tolerance using Bacillus megaterium
10645936 Bacillus megaterium bioactive compositions and metabolites
10683470 Product for cleaning, sanitizing and hygienization
4332898 Hybrid plasmid and process of making same
4427771 Assay method for amylase activity and method of producing maltose dehydrogenase for use therein
4542098 Production of glucose dehydrogenase and use of the resultant enzyme in the enzymatic synthesis of L-carnitine
4554250 Method of increased production of penicillin acylase and plasmid employed therein
4935359 Fermentation process
5030574 Plasmidless Lac strain of bacilus megaterium QM B1551